Concentration Assay:Article Title: Baricitinib ameliorates skin fibrosis via direct fibroblast suppression and endothelial exosome-mediated paracrine signaling.
Article Snippet: .. Establishment of fibrosis model in HSFs To determine the optimal concentration of bleomycin sulfate (BLM; #HY-17565, MCE) for inducing fibroblast activation, HSFs were treated with various concentrations of BLM (0, 5, 10, 20, 40, and 80 ng/mL) for different durations (24, 48, and 72 hours), as previously described [15]. .. To investigate the effects of JAK inhibition on BLM-induced HSF proliferation and fibrosis, HSFs were first treated with 40 ng/mL BLM for 24 hours, followed by incubation with Baricitinib (#HY-15315, MCE) at varying concentrations (1, 3, 6, 12, and 24 nmol/L)..
Activation Assay:Article Title: Baricitinib ameliorates skin fibrosis via direct fibroblast suppression and endothelial exosome-mediated paracrine signaling.
Article Snippet: .. Establishment of fibrosis model in HSFs To determine the optimal concentration of bleomycin sulfate (BLM; #HY-17565, MCE) for inducing fibroblast activation, HSFs were treated with various concentrations of BLM (0, 5, 10, 20, 40, and 80 ng/mL) for different durations (24, 48, and 72 hours), as previously described [15]. .. To investigate the effects of JAK inhibition on BLM-induced HSF proliferation and fibrosis, HSFs were first treated with 40 ng/mL BLM for 24 hours, followed by incubation with Baricitinib (#HY-15315, MCE) at varying concentrations (1, 3, 6, 12, and 24 nmol/L)..
Staining:Article Title: Inhalable TFRC-Targeted Extracellular Vesicles Delivery of siTGF-β1 Alleviates Pulmonary Fibrosis via Dual Inhibition of Ferroptosis and Fibroblast Activation
Article Snippet: Six- to eight-week-old male C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and maintained at a constant temperature (25 ± 1 °C). .. DMEM culture medium, penicillin-streptomycin (PS) and Fetal bovine serum (Gibco), Bleomycin sulfate, Fer-1 and DFO (MCE), HE staining kit, Masson’s staining kit and 0.5% crystal violet staining solution (Yeasen), EPPB enhanced Prussian blue staining kit and FITC-Phalloidin (Solarbio), Transwell (Corning), CCK-8 and 4% PFA (Biosharp), C11-BODIPY581/591 (Merck), RhoNox-1, Deferoxamine (DFO), Bleomycin sulfate and Liproxstatin-1 (MCE), Dihydroethidium (Beyotime), BC-T4 culture medium (Baso), anti-CD34, anti-CD19 and anti-CD45 (BD), anti-CD90, anti-CD73 and anti-CD105 (Biolegend), anti-CD63, anti-Flotillin-1, anti-Calnexin and anti-TGF-β1 (HUABIO), anti-Collagen I (Abcam), anti-TFRC (Abways), anti-α-SMA (Proteintech), Recombinant Mouse/Rat TGF-beta 1 (Novoprotein, Shanghai, China). ..
Article Title: Inhalable TFRC-Targeted Extracellular Vesicles Delivery of siTGF-β1 Alleviates Pulmonary Fibrosis via Dual Inhibition of Ferroptosis and Fibroblast Activation
Article Snippet: Six- to eight-week-old male C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and maintained at a constant temperature (25 ± 1 °C). .. Materials and Instruments DMEM culture medium, penicillin-streptomycin (PS) and Fetal bovine serum (Gibco), Bleomycin sulfate, Fer-1 and DFO (MCE), HE staining kit, Masson’s staining kit and 0.5% crystal violet staining solution (Yeasen), EPPB enhanced Prussian blue staining kit and FITC-Phalloidin (Solarbio), Transwell (Corning), CCK-8 and 4% PFA (Biosharp), C11-BODIPY581 /591 (Merck), RhoNox-1, Deferoxamine (DFO), Bleomycin sulfate and Liproxstatin-1 (MCE), Dihydroethidium (Beyotime), BC-T4 culture medium (Baso), anti-CD34, anti-CD19 and anti-CD45 (BD), anti-CD90, anti-CD73 and antiCD105 (Biolegend), anti-CD63, anti-Flotillin-1, anti-Calnexin and anti-TGF-β1 (HUABIO), anti-Collagen I (Abcam), antiTFRC (Abways), anti-α-SMA (Proteintech), Recombinant Mouse/Rat TGF-beta 1 (Novoprotein, Shanghai, China). ..
CCK-8 Assay:Article Title: Inhalable TFRC-Targeted Extracellular Vesicles Delivery of siTGF-β1 Alleviates Pulmonary Fibrosis via Dual Inhibition of Ferroptosis and Fibroblast Activation
Article Snippet: Six- to eight-week-old male C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and maintained at a constant temperature (25 ± 1 °C). .. DMEM culture medium, penicillin-streptomycin (PS) and Fetal bovine serum (Gibco), Bleomycin sulfate, Fer-1 and DFO (MCE), HE staining kit, Masson’s staining kit and 0.5% crystal violet staining solution (Yeasen), EPPB enhanced Prussian blue staining kit and FITC-Phalloidin (Solarbio), Transwell (Corning), CCK-8 and 4% PFA (Biosharp), C11-BODIPY581/591 (Merck), RhoNox-1, Deferoxamine (DFO), Bleomycin sulfate and Liproxstatin-1 (MCE), Dihydroethidium (Beyotime), BC-T4 culture medium (Baso), anti-CD34, anti-CD19 and anti-CD45 (BD), anti-CD90, anti-CD73 and anti-CD105 (Biolegend), anti-CD63, anti-Flotillin-1, anti-Calnexin and anti-TGF-β1 (HUABIO), anti-Collagen I (Abcam), anti-TFRC (Abways), anti-α-SMA (Proteintech), Recombinant Mouse/Rat TGF-beta 1 (Novoprotein, Shanghai, China). ..
Article Title: Inhalable TFRC-Targeted Extracellular Vesicles Delivery of siTGF-β1 Alleviates Pulmonary Fibrosis via Dual Inhibition of Ferroptosis and Fibroblast Activation
Article Snippet: Six- to eight-week-old male C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and maintained at a constant temperature (25 ± 1 °C). .. Materials and Instruments DMEM culture medium, penicillin-streptomycin (PS) and Fetal bovine serum (Gibco), Bleomycin sulfate, Fer-1 and DFO (MCE), HE staining kit, Masson’s staining kit and 0.5% crystal violet staining solution (Yeasen), EPPB enhanced Prussian blue staining kit and FITC-Phalloidin (Solarbio), Transwell (Corning), CCK-8 and 4% PFA (Biosharp), C11-BODIPY581 /591 (Merck), RhoNox-1, Deferoxamine (DFO), Bleomycin sulfate and Liproxstatin-1 (MCE), Dihydroethidium (Beyotime), BC-T4 culture medium (Baso), anti-CD34, anti-CD19 and anti-CD45 (BD), anti-CD90, anti-CD73 and antiCD105 (Biolegend), anti-CD63, anti-Flotillin-1, anti-Calnexin and anti-TGF-β1 (HUABIO), anti-Collagen I (Abcam), antiTFRC (Abways), anti-α-SMA (Proteintech), Recombinant Mouse/Rat TGF-beta 1 (Novoprotein, Shanghai, China). ..
Recombinant:Article Title: Inhalable TFRC-Targeted Extracellular Vesicles Delivery of siTGF-β1 Alleviates Pulmonary Fibrosis via Dual Inhibition of Ferroptosis and Fibroblast Activation
Article Snippet: Six- to eight-week-old male C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and maintained at a constant temperature (25 ± 1 °C). .. DMEM culture medium, penicillin-streptomycin (PS) and Fetal bovine serum (Gibco), Bleomycin sulfate, Fer-1 and DFO (MCE), HE staining kit, Masson’s staining kit and 0.5% crystal violet staining solution (Yeasen), EPPB enhanced Prussian blue staining kit and FITC-Phalloidin (Solarbio), Transwell (Corning), CCK-8 and 4% PFA (Biosharp), C11-BODIPY581/591 (Merck), RhoNox-1, Deferoxamine (DFO), Bleomycin sulfate and Liproxstatin-1 (MCE), Dihydroethidium (Beyotime), BC-T4 culture medium (Baso), anti-CD34, anti-CD19 and anti-CD45 (BD), anti-CD90, anti-CD73 and anti-CD105 (Biolegend), anti-CD63, anti-Flotillin-1, anti-Calnexin and anti-TGF-β1 (HUABIO), anti-Collagen I (Abcam), anti-TFRC (Abways), anti-α-SMA (Proteintech), Recombinant Mouse/Rat TGF-beta 1 (Novoprotein, Shanghai, China). ..
Article Title: Inhalable TFRC-Targeted Extracellular Vesicles Delivery of siTGF-β1 Alleviates Pulmonary Fibrosis via Dual Inhibition of Ferroptosis and Fibroblast Activation
Article Snippet: Six- to eight-week-old male C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and maintained at a constant temperature (25 ± 1 °C). .. Materials and Instruments DMEM culture medium, penicillin-streptomycin (PS) and Fetal bovine serum (Gibco), Bleomycin sulfate, Fer-1 and DFO (MCE), HE staining kit, Masson’s staining kit and 0.5% crystal violet staining solution (Yeasen), EPPB enhanced Prussian blue staining kit and FITC-Phalloidin (Solarbio), Transwell (Corning), CCK-8 and 4% PFA (Biosharp), C11-BODIPY581 /591 (Merck), RhoNox-1, Deferoxamine (DFO), Bleomycin sulfate and Liproxstatin-1 (MCE), Dihydroethidium (Beyotime), BC-T4 culture medium (Baso), anti-CD34, anti-CD19 and anti-CD45 (BD), anti-CD90, anti-CD73 and antiCD105 (Biolegend), anti-CD63, anti-Flotillin-1, anti-Calnexin and anti-TGF-β1 (HUABIO), anti-Collagen I (Abcam), antiTFRC (Abways), anti-α-SMA (Proteintech), Recombinant Mouse/Rat TGF-beta 1 (Novoprotein, Shanghai, China). ..
other:Article Title: CLIC3 promotes lung cancer cell proliferation by regulating intracellular chloride and ROS levels.
Article Snippet: Lung cancer is one of the most prevalent and lethal malignancies worldwide, ranking among the leading causes of cancer-related morbidity and mortality.. Uncontrolled cellular proliferation constitutes its central pathological hallmark, underscoring the critical need to identify novel, druggable therapeutic targets.. Recent studies have demonstrated that chloride intracellular channel 3 (CLIC3), a multifunctional protein exhibiting both ion channel activity and oxidoreductase function, may play pivotal roles in tumorigenesis.
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